摘要
以水杨酸(SA)、FeSO4、K2HPO4、MgCl2、SA FeSO4、SA MgCl2 为诱抗剂,测定了新疆彩色棉花不同品种喷施诱抗剂后再接种枯萎菌幼苗叶片的叶绿素含量,PAL、CAT酶活性,PR蛋白的表达及信号物质SA含量的变化。结果表明,诱导后挑战接种,叶绿素含量、PAL、CAT酶活性都有增加,且耐病品种高于感病品种。经SDS PAGE电泳分析,叶片中有数种病程相关蛋白(PR蛋白)表达,信号物质SA含量呈先上升后下降的变化趋势。
According to this present situation, the promising way is inducing the plant acquired the SAR by inducer. It has some advantages,e.g.safe,efficient,wide and last etc. Inducing resistance is a kind of hereditary potential of plant.And the potential can be expressed if we use appropriate inducer,which can strengthen the ability of resistance against germs,fungi and viruses. We studied different varities of colored cotton in Xinjiang, and screened out several effectivley inducers. Having used SA, FeSO_4,K_2HPO_4, MgCl_2,SA-FeSO_4 , SA-MgCl_2 , we induced two species:Xincaimian1 and Xincaimian3. Then searched the change of different resistance substance in leaves after infection with Fuserium oxysporum . The result showed great changes have taken place in the content of relatively resistance substance after inducement and challenge.There are two parts.(1) the content of chlorophyll , and activity of phenylalanine ammonia lyase(PAL) & calatase(CAT) in contrast to control have remarkably increased.And,Xincaimian1,a resistant cultivar,showed a higher increasing rate of them than a susceptible cultivar,Xincaimian3. (2)After inducement ,the change of SA,which is possible signal in SAR,can be detected in two varities of colored cotton.And it showed the changing trend from increasing to decreasing. With the increasing of content of SA, pathogenesis-related-proteins(PR Proteins) were induced,which can promote the resistance of colored cotton. From above,it is feasible that inducer can increase the resistance against Fuserium oxysporum. The effect of mixture inducers was much better than single ones among the six kinds of inducer.
出处
《棉花学报》
CSCD
北大核心
2005年第2期107-111,共5页
Cotton Science
基金
国家自然科学基金(30240077)
