家兔抗着床实验中炔诺酮肟对子宫内膜影响的形态学研究

Morphological Studies of Effect of Norethisterone Oxime on Endometrium in Anti-implantation Experiments on Rabbits

家兔从妊娠第1天开始,每天灌服炔诺酮肟4mg/kg,连续3天,第9天处死。取子宫作HE、PAS、ACP、AKP及SDH染色。结果表明,炔诺酮肟呈明显的抗着床作用。光镜观察,子宫内膜呈退行性改变,蜕膜化减弱。组织化学染色,AKP活性减弱(P<0.05),糖原含量下降(P<0.01),ACP及SDH活性无明显变化。提示:给药后子宫内膜生长发育受抑制、代谢降低,致使胚泡与子宫内膜不能同步,可能与抗着床机理有关。

The rabbits were administered orally for 3 days 4 mg/kg·day norethis-terone oxime(NETO) after successful coitus and sacrificed on the 9th day. The uteri were dissected for histological examination after being stained by HE, PAS, ACP, AKP and SDH. It was found that NETO exerted obvious anti-implantation effect. Under light-microscopic examination regressive changes were observed, with less obvious decidualization. Upon histochemical observation, the activity of AKP decreased, the glucogen content decreased, while ACP and SDH underwent no significant changes. The results indicate that the growth and development of endometrium are inhibited, and metabolism depressed, thus causing desynchronization between the embryo and the endometrium. These might be related to anti-implantation mechanism of NETO.