摘要
目的 从行为学和红核神经元数目等角度来探讨电针对脊髓损伤后神经组织保护作用的机理。方法 应用ALLEN’s致伤法制成大鼠T1 2 中度脊髓损伤模型 ,设立电针组进行治疗 ,并同时设立正常组、假手术组、单纯针刺组和造模组作为对照 ;应用斜板实验对不同组的大鼠在造模后 12h、1星期和 4星期作行为学评分 ,4星期时 ,利用HRP逆行示踪技术 ,计数各组每个大鼠红核神经元数目。结果 在脊髓损伤后 12h和 1星期 ,电针组大鼠与单纯针刺组和造模组大鼠相比 ,在斜板实验方面无明显差异 ,在脊髓损伤后 4星期 ,电针组大鼠在斜板实验方面明显高于单纯针刺组和造模组大鼠 ,在斜板实验方面 ,假手术组与正常组只在伤后 12h有差别 ,在伤后 1星期和 4星期 ,两组无明显差别。同时 ,在脊髓损伤后 4星期 ,电针组大鼠的红核神经元数目明显高于单纯针刺组和造模组大鼠的红核神经元数目。结论 电针治疗能促进大鼠后肢功能的恢复 ,提高其行为学评分 。
Objective To investigate the mechanism of the protective action of electroa(upuncture on nervous tissues after spinal injury from behavior and the number of red nucleus neurons.Methods A rat model of moderate T 12 spinal injury was made by ALLEN’s injury-inducing method. A electroacupuncture group was established and treated. Meanwhile normal, sham operation, simple acupuncture and model groups wre established as controls. The behavior was scored by a slant-board test at 12 hrs, 1 week and 4 weeks after model making in different groups of rat. At 4 weeks red nucleus neurons was counted by HPR antidromic tracer technique in each rat of different groups.Results At 2 hrs and 4 week after spinal injury, the slant-board test score did not vary significantly in the electroacupuncture group as compared with the simple acupuncture and model groups. At 4 weeks after spinal injury, the slant-board test score was significantly higher in the electroacupuncture group than in the simple acupuncture and model groups. Only at 12 hrs after the injury was there a significant difference in slant-board test score between the sham operation and normal groups and there was not at 1 week and 4 weeks after. At 4 week after spinal injury the number of red nucleus neurons was significantly larger in the electroacupuncture group than in the simple acupuncture and model groups. Conclusion Electroacupuncture treatment can promote the recovey of rat's hind leg function, increase its behavior score and reduce antidromic injury to red nucleus neurons.
出处
《上海针灸杂志》
2005年第3期37-39,共3页
Shanghai Journal of Acupuncture and Moxibustion
关键词
电针
脊髓损伤
行为学
红核神经元
Electroacupuncture
Spinal injury
Behavior study
Red nucleus neurons