植物源食品DNA制备方法的比较研究

DNA Extraction from Genetically Modified Foods by Modified CTAB Method or SDS Method

目的:探索可用作PCR方法检测和鉴定植物源转基因食品模板的DNA抽提方法。方法:分别用改良CTAB法、SDS法制备黄豆、玉米、土豆和番茄及其加工产品的DNA,PCR扩增叶绿体基因片段及黄豆、玉米的内参照基因lectin和zein10。结果:改良CTAB法所得DNA作为PCR扩增模板,叶绿体基因片段及lectin和zein10均呈阳性,SDS法所得DNA作为模板时,部分样品内参照基因lectin或zein10扩增阴性。结论:PCR方法检测转基因食品时,应用改良CTAB法制备DNA模板。

Object: To explore the DNA extraction methods for preparing DNA suitable for PCR-based detection of genetically modified organisms in foods and animals feed. Methods: DNAs of soybean, maize, tomato, potato and the processed products from these materials were extracted by using modified CTAB method or SDS method, and then were used as templates for PCR amplifying trnL gene of chloroplast as well as lectin gene of soybean and zein 10 gene of maize. Results: trnL gene of chloroplast and lectin gene of soybean and zein 10 gene of maize were revealed positive by PCR when using DNAs extraction by modified CTAB method as templates, while lectin and zein 10 were revealed negative by PCR amplifying of several DNA samples prepared by SDS method. Conclusion: DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed.