玫瑰茄红色素主要呈色物质分离提纯的研究

Study on Extraction and Purification of Roselle Calyx Main Red Pigment

本文用纸层析法对玫瑰茄红色素主要呈色物质进行分离提纯,结果表明:玫瑰茄红色素是一种花青甙类色素,纸层析出现紫红、浅红、浅橙三个色斑,紫红色为玫瑰茄红色素主要呈色物质,其R f为0.203。玫瑰茄红色素纸层析最佳展开剂为正丁醇:冰醋酸:水=4:1:1,最佳洗脱剂为0.1%稀盐酸,紫红色色素的粗提取率为0.704%,占色素总含量的46.93%。

In this paper,the Roselle calyx red pigment was extracted and purified by paper chromatography. The results showed that the Roselle calyx pigment was a kind of anthocyanin. The pigment would appear in purplish red, light red and light orange colour spots when the pigment was analysed by paper chromatography. Purplish red colour spots were the major pigment when the Rf was 0.203. The optimum developer was CH3(CH2)3OH:HAC:H2O=4:1:1 and the optimum eluent was 0.1% HCl. The extraction rate of the purplish red pigment was 0.704%, accounted for 46.93 per cent of the total pigment content.