鼠尾藻多糖的制备及其抗氧化活性

Preparation of Polysaccharide Derived from Sargassum thunbergii and Its Antioxidant Activity

采用正交试验设计,得到了鼠尾藻多糖的最佳提取条件:pH=4,破壁功率600W,破壁时间200s,提取温度100℃,提取时间6h,三氯乙酸浓度3%(W/V),乙醇浓度70%(V/V);在此条件下,多糖提取率可达到6.24%(W/W)。通过Sepharose4B柱对该多糖进行了层析纯化,并用自由基清方法测定了其体外抗氧化活性,结果表明:鼠尾藻多糖有较高的抗氧化活性,对DPPH·的清除率在50%以上,对·OH和O■·直接清除能力也超过了30%。

Through an orthogonal design L16(45), the optimized preparing condition of polysaccharide from Sargassum thunbergii was found at pH=4, applying ultrasound at 800W for 200s, incubating at 100℃ for 6h, and 3% (W/V) of trichloroacetic acid and 70% (V/V) of ethanol. The extraction yield of polysaccharide was up to 6.24%. The crude polysaccharide was purified with Sepharose 4B column chromatography and the antioxidant activity of polysaccharide from Sargassum thunbergii was determined by radical scavenging methods. The result showed that the polysaccharide had satisfactory antioxidant activity: the scavenging rate was more than 50% towards DPPH·, and was also more than 30% towards ·OH and O■·