摘要
将银杉种子的成熟胚剥离出来，在ＬＰ＋ＮＡＡ１．５×１０￣（－６）培养基中离休培养，成苗率为８７％。以无菌幼苗的子叶、下胚轴、顶芽作为外植体，在ＬＰ附加ＫＴ０．５，ＢＡ０．５，ＮＡＡｌｘ１０￣（－６）的培养基中诱导出愈伤组织。这一研究为银杉离体胚培养进行快速繁殖，作了有益的尝试。
he mature embryos of Cathaya argyrophylla Chun et Kuang were removed fromseeds and cultured on LP＋NAA 0.5～2.0 × 10^(-6)media. The rate of seedling is 87％ on themedium contained NAA 1.5 × 10^(-6).The cutting segments of sterile cotyledon,apical budand hypocotyl were cultured on LP medium contained KT 0.5.BA 0.5. NAA 1 × 10^(-6)andthe callus was formed after 3 weeks.
出处
《西南林学院学报》
1994年第3期155-158,共4页
Journal of Southwest Forestry College
关键词
银杉
离体胚培养
愈伤组织
诱导
Cathaya argyrophylla
Excised embryo culture
Callus
