山羊发情周期的人工模拟实验

Artificial Oestrus Cycle in the Goat

将２８只摘除卵巢后６～７周的山羊随机分为４组，按２×２因子实验设计进行处理，实验因子为：①肌肉注射孕酮（Ｐ＿４）：１～５天每天１０ｍｇ；６～１２天每天２０ｍｇ．②肌肉注射雌二醇（Ｅ＿２）：第１３天２０ｕｇ；第１４天４０ｕｇ．对照处理则注射相应体积的玉米油，在第１～１５天每天采１次颈静脉血样，用ＲＩＡ法测定血浆Ｐ＿４和Ｅ＿２水平，结果表明，每天注射１０和２０ｍｇＰ＿４可使血浆Ｐ＿４水平分别维持在３和５ｎｇ／ｍｌ左右，注射２０和４０μｇＥ＿２可分别产生３０和５０ｐｇ／ｍｌ的血浆Ｅ＿２水平。用这种方法，简便而准确地模拟了山羊发情周期中血浆Ｐ＿４和Ｅ＿２水平的变化范型。

A 2 × 2 factorically designed experiment was conducted. Twenty-eight goatsovariectomized 6～7 weeks previously were randomly divided into 4 groups and each group was givendifferent treatment. One treatment factor was P_4 injection : 10 mg P_4/goat from the lst day to the 5thday and 20 mg P_4 from the 6th day to the 12th day Another factur was E_2 injection: 20 ug E_2/goat onthe 13th day and 10 ug E_2 on the 14th day Jugular blood samples were collected once a day from thelst day to the 15th day and plasnia levels of P_4 and E_2 were measured with RIA. Injection of 10 and20 mg P_4 each day maintained the plasma levels of P_4 at 3 and 5 ng/ml respectively and administrationof 20 and 40 ug E_2 each day preduced the plasma levels uf E_2 at 30 and 50 pg/ml respectively. Thiswas a simple method for artificial oestrus cycle with sex steroids in the goat.