高效液相色谱法测定妇乐颗粒中延胡索乙素的含量

Determination of Tetrahydropalmatione in FuLe Granules by HPLC

目的建立妇乐颗粒中延胡索乙素的含量测定方法。方法采用HPLC法,流动相:甲醇-0.1%磷酸(用三乙胺调pH至6.0)(60∶40),色谱柱:DiamonsilTMC18(250×4.6mn,5μm),流速:1.0mL/min,柱温:35℃,波长:280nm,进样量:20μL。结果延胡索乙素在0.102～1.632μg范围内线性良好,平均回收率为98.78%,RSD为2.75%。结论该方法简便、可靠,可用于妇乐颗粒的质量控制。

Objective To establish a method for the determination of tetrah yd ropalmatione in FuLe granules. Methods A HPLC method was used with DiamonsilTM C 18(250× 4.6 mn, 5 μ m) column and a mobile phase of methanol- 0.1 % phospho ric acid solution (adjusted the pH to 6.0 with triethylamine)(60 ∶ 40). Flow ra te was 1.0 mL/min, column tem perature 35 ℃ , the detection wavelength at 280nm and sample loop volume bei ng 20 μ L. Results The calibration curve was linear within the range of 0.102 ～ 1.632 μ g of tetrahydropalmatione, the average recovery was 98.78 % (RSD=2 .75 % ).Conclusion The method is simple and accurate.It can be used for the q uality control of Fule granules.