PP_（333）浸种对油菜幼苗抗寒性的生理效应

PHYSIOLOGICAL EFFECT OF PP_(333) WITH DIFFERENT CONCENTRATIONS UPON COLD RESISTANCE OF RAPE SEEDLINGS

用ＰＰ（３３３）浸种后的油菜幼苗，经４℃、３６小时低温胁迫处理，发现ＰＰ（３３３）能阻止幼苗的电解质渗漏，提高其超氧化物歧化酶（ＳＯＤ）和过氧化氢酶（ＣＡＴ）活性，降低丙二醛（ＭＤＡ）含量，减缓低温对幼苗叶绿素的破坏，提高可溶性蛋白质含量和抗坏血酸（Ｖｃ）含量。ＰＰ（３３３）对油菜幼苗游离脯氨酸含量无明显作用。ＰＰ（３３３）的较适处理浓度为０．１—０．５μｇ／ｍｌ，以０．５μｇ／ｍｌ为佳。

Seedlings from seeds soaked with PP(333) were put in low temperature (4℃) stress conditions for 36 hours. It was able to prevent electrolyte leakage, increase the activity of SOD and CAT, decrease MDA content, reduce the injury caused by low temperature in chlorophyll, increase contents of soluble protein and ascorbic acid. PP(333) had no effect on content of free proline. The selected concentrations of PP(333) were 0.1-0.5μg/ml, and 0.5μg/ml was the optimum concentration.