影响甘蔗胚性愈伤组织原生质体培养的主要因素

PRIMARY FACTORS INFLUENCING PROTOPLASTS CULTURED FROM EMBRYOID CALLUS IN SUGARCANE

以甘蔗胚性意伤组织作原生质体培养材料，对影响甘蔗胚性意伤组织原生质体培养的因素进行了研究。结果表明，其影响因素主要有：材料的基因型；酶解前的培养基配方；原生质体培养基种类；植物生长调节剂浓度及其配比和不同培养方式等。其中，影响原生质体形成愈伤组织的最大因素是材料的基因型。５３％的参试基因型材料发生了分裂，部分形成了细胞团，其中有两个材料（一为栽培材料，一为野生资源材料）的原生质体细胞经进一步培养发育成为愈伤组织。

The influential factors for protoplast culture were investigted using embyogenic calli of sugarcane as materials. It wasfound that the main influential factors included genotype of the mainals,culture medium before isolation,type of protoplastculture medium, different methods, concentration and constitution of exogenous hormones etc. Among these factors, thegenotype of materiaes played mest important role in forming calli. 53% of genotypic material occured division,part of themformed cell colonies. Two cell colonies derived from ptotoplast occured sustaining division and formed calli.