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酶法合成高比活度[α-^(32)P]dATP

ENZYMATIC SYNTHESIS OF [α—^(32)P]dATP OF HIGH SPECIFIC ACTIVITY
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摘要 本文介绍了通过一系列连续的酶促反应制备高比活度[α-^(32)P]dATP的方法。该法简便、不需要中间产物的分离纯化、操作放射性步骤少、制备周期短,产品用高压液相制备色谱分离纯化,效率高、纯度在98%以上。用该法制备的[α-^(32)P]dATP比活度高,能够满足遗传工程研究中进行DNA缺口翻译标记及分子杂交等工作的需要。 This report desceibed a method for enzymaticpreparation of [α-^(32)P] dATP of high specific activity through a series of enzymatic reactions. The method is simple and convenient, transfer and purification of intermediate products is not necessary. It reduces manipulations of large amounts of radioactivity. The product was isolated by preparation HPLC. Its radiochemical purity is higher than 98%. The [α-^(32)P] dATP of high specific activity prepared by enzymatic method is satisfactorily used for the labelling of DNA (probe) by nick translation and'molecular hybridization.
出处 《同位素》 CAS 北大核心 1989年第3期150-154,共5页 Journal of Isotopes
关键词 磷32 dATP 缺口翻译 分子杂交 [α-^(32)P]dATP, nick translation, molecular hybridization
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