杨木粉酶水解工艺条件的研究

STUDY ON TECHNIC CONDITIONS FOR ENZYMATIC HYDROLYSIS OF POPLAR WOOD POWDER

研究了杨木粉酶水解的工艺条件。实验结果表明丙酸处理杨木粉的最优条件为: 900g/L的丙酸, 3g/L的盐酸, 121℃蒸解 90min;处理过的杨木粉用纤维素酶水解,酶解温度 50℃、底物浓度 50g/L、每 100g底物加酶 1. 00mL、水解时间 72h,葡萄糖浓度可达 22. 5g/L。

This paper presents technology conditions of enzymatic hydrolysis of poplar wood powder. The pretreatment conditions are 900 g/L propionic acid, 3 g/L HCl, reaction temperature 121 ℃, reaction time 90 minutes. The best conditions of enzymatic hydrolysis are 50 ℃ of enzymatic hydrolysis temperature, 1.00 mL cellulose per 100 g (substrate), 50 g/L of substrate concentration and 72 hrs of hydrolysis duration.The glucose content may reach 22.5 g/L.