产肌酐水解酶菌株的分离、鉴定及产酶条件研究

Isolation, Identification of Creatininase Producing Bacteria and Study on the Conditions for the Enzyme Production

研究分离到几株产肌酐水解酶的菌株,对所产酶的特性分析表明,菌株 42 1 表现较高的产酶性能且所产的酶具有高的热稳定性.对菌株42 1产酶发酵条件的研究表明,菌株 42 1 以酵母膏和玉米浆为氮源时表现较高的产酶性能,葡萄糖等容易利用的碳源的存在对酶的产生没有抑制作用.诱导物肌酐、肌酸、肌氨酸、氯化胆碱和尿素可以诱导酶的产生,Mn2+、Co2+对酶的产生有促进作用.

Bacteria capable of producing creatininase were isolated from soil. Strain 42-1 showed higher yield of creatininase that was more heat-resistant than that of others. Studying on the conditions for fermentation showed that higher enzyme activity was obtained using yeast extract and corn-steep liquor as nitrogen sources. The easily used carbon sources such as glucose had no catabolic repression effect on the creatininase production. Creatininase could be induced by the reagents such as creatinine, creatine, sarcosine, choline chloride and urea. Mn~(2+_、Co~(2+_ could remarkably increased the productivity of creatininase.