西藏小麦品种资源HMW-GS组成研究

Analysis on the Components of HMW-GS of Tibet Wheat Variety Resources

为了给小麦品质改良提供基础材料,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法,分析了西藏地方品种、西藏主要育成品种和近几年从国内外引进品种(系)等805份小麦的高分子量谷蛋白亚基(HWM-GS)组成。结果表明,Glu-1位点共有24种等位基因,其中Glu-A1位点4种、Glu-B1位点10种、Glu-D1位点10种;亚基N、7+8和2+12在各自位点的频率最高,分别为66.70%、40.25%和59.88%;另外,在Glu-D1位点,还发现有了稀有亚基2.2+12,其频率为0.62%。引进品种的2*、14+15、17+18和5+10等优质亚基出现的频率较西藏主要育成品种高,西藏主要育成品种中优质亚基5+10的频率仅为0.24%,西藏品种亚基组合类型主要为N,7+8,2+12。

In order to provide basic (materials) for improving wheat quality, 805 (Tibet) wheat (varieties) were analyzed with SDS-PAGE on the alleles (variance) of high molecular weight gluten in gluten subunits (HWM-GS), some of the wheat (varieties) were local (varieties) and some (varieties) (lines) were from out of (Tibet). The (result) showed that there were 24 alleles on Glu-1 loci, 4 alleles on Glu-A1 loci, 10 alleles on Glu-B1 loci, 10 alleles on Glu-D1 loci. The (frequencies) of N, 7+8, 2+12 alleles gene were high on the Glu-A1, Glu-B1 and Glu-D1 loci, (respectively), and were 66.70%, 40.25%, 59.88%. The (frequencies) of elite (subunits) of 2~*, 14+15, 17+18 and 5+10 of some (varieties) (lines) out of (Tibet) was higher than that of (Tibet) (varieties), and some rare subunit types such as 2.2+12 (subunit) in Glu-D1 loci among them were found. The (frequency) of elite (subunits) 5+10 was only 0.24% among (Tibet) main (varieties).