结核病患者免疫学指标检测的临床意义

Clinical Significance of Detection of Immunological Marker in Tuberculosis Patients

目的 :探讨结核病患者免疫学指标检测的临床意义。方法 :采用流式细胞术、散射比浊法和 EL ISA分别检测了 5 6例结核病患者和 32例健康人的 T淋巴细胞亚群、自然杀伤细胞、血清免疫球蛋白及其亚类、白细胞介素 - 6和白细胞介素 - 2受体。结果 :结核病患者 CD3+细胞与对照组无差别 (P>0 .0 5 ) ,但 CD8+细胞明显高于对照组 (P<0 .0 5 ) ,CD4 +/CD8+明显低于对照组 (P<0 .0 5 ) ;自然杀伤细胞高于对照组血清 Ig G、Ig M和 Ig A含量与对照组基本无区别 (P>0 .0 5 ) ,但 Ig G亚类差别较大 ,尤其是 Ig G1和 Ig G2 (P<0 .0 1)血清 IL - 6和 IL - 2 R明显高于对照组。结论 :机体的细胞免疫和体液免疫均参与了结核病的发生、发展过程。

Objective:To explore clinical significance of detection of immunological marker in tuberculosis patients.Methods:The T lymphocyte subtypes, nature killing cells,immunoglobins and their subtypes,IL-6 and sIL-2R were detected by flow cytometry,nephelometric assay and ELISA,respectively.Results:In T lymphocyte subtypes,there was no difference in CD3+ subpopulation, but significant difference in CD8+ subpopulation and nature killing cells; there was no difference in serum concentration of immunoglobins, but significant difference in immunoglobin G subtypes,espcialy in IgG1 and IgG2.Conclusion:Cellular immunity and humoral immunity were both involved in the occurrence and development of tuberculosis.