宁波市2002～2004年流行性感冒的检测与HA1序列分析

Monitoring of influenza virus and comparison of the HA1 sequence in Ningbo from 2002 to 2004

目的:为了解近年来宁波市流行性感冒的流行情况以及病毒抗原性的变异情况.方法:采用MDCK细胞培养和鸡胚培养法分离流感病毒,交叉血凝抑制法进行抗原分析,对甲3型流感病毒的HA1血凝素基因作了核苷酸序列测定.结果:2002年～2004年共分离到流感病毒330株,其中甲3型324株(98.2%)、甲1型1株(0.3%)、乙型5株(1.5%).甲3型毒株2002年与2003年及2004年的抗原比分别为1.9和2.45,核苷酸序列测定结果2002年～2004年HA1区约以5个氨基酸改变的速度变异.结论:3年来宁波市一直以甲3型流感为主要流行株,甲1型及乙型流感则以散发存在.甲3型流感病毒虽说没有发生重大变异,但其抗原性每年都有一定的漂移.

Objective:To understand the epidemics and antigenic drift of influenza viruses in Ningbo in 2002～2004.Methods: Influenza viruses were isolated with the means of cultured in MDCK cell and embryonated eggs. The cross hemagglutination inhibition test was used for antigenic analysis and determined nucleotide sequence of HA1 genes of the influenza A (H3N3) virus.Results: 330 influenza strains were obtained, including 324(98.2%)strains of H3N2, 1(0.3%)strains of H1N1 and 5(1.5%)strains of B. The antigenic ratio of H3N2 was 1.9 between 2002 strains and 2003 strains, and 2.45 between 2002 strains and 2004 strains. Nucleotide sequence analysis indicated the variant rate of HA1 from 2002 to 2004 was 5 amino acids per year.Conclusion: The influenza A (H3N3) virus prevailed in the past 3 years in Ningbo, influenza A (H1N1) and influenza B were presence with the quomodo of disperse. Although it was no oversize gene variation, influenza A (H3N3) virus antigenicity appreciably drifts annually. [