影响青花菜游离小孢子培养的若干因素

Several factors affecting isolated microspore culture in broccoli

10个青花菜杂种游离小孢子培养有 6个诱导出胚状体,诱导率达 60%,其中绿洲 808产胚量可达 13. 6个·蕾 -1;小孢子处于单核晚期到双核期适宜小孢子培养;在 1 /2NLN培养基中添加适量的蔗糖、活性炭、6 BA、AgNO3、2, 4 D有利于胚胎发生; 32℃热激处理 1d有利于小孢子产生胚状体;采蕾前 2-5d的温度在 15-25℃适合小孢子发育.

Isolated microspores of ten different broccoli hybrids were cultivated; embryoids had been obtained from six hybrids, the inducement ratio up to 60%. Among these hybrids, Luzhou 808 had 13.6 embryos per bud. The optimum stage for the microspore cultivation was from uninucleate anaphase to dikaryolization phase. It was advantageous for embryo inducement by adding suitable amount of sucrose, activated carbon, 6-BA, AgNO_3 and 2,4-D into medium 1/2NLN. The heat-shock treatment at temperature 32 ℃ for 24h had a positive effect on the embryogenesis. While 2-5 days before plucking buds, the temperature between 15 ℃ and 25 ℃ was suitable for the microspore growth.