奶牛乳铁蛋白基因启动子区PCR-RFLP分析与乳房炎的相关性

Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with mastitis

采用CMT方法检测奶牛的乳房炎发病情况.筛选 90头分别设为对照组(健康奶牛)、隐性乳房炎组 (试验组Ⅰ )和临床乳房炎组(试验组Ⅱ),每组 30头.检测每头奶牛的NAGase活性,并用限制性片段长度多态性 (RFLP)分析技术,检测乳铁蛋白(Lf)基因启动子区域的RFLP多态性.结果表明:不同组别的奶牛之间NAGase活性差异显著 (P<0. 01),且Lf基因启动子区域存在RFLP多态性,说明该多态性与乳房炎存在一定关系,可能是奶牛乳房炎的一个分子标记.

CMT was used to detect the incidence of mastitis. Ninety cows were selected and allotted into 3 groups, 30 animals in each group: control group (healthy cows), experimental groupⅠ (cows with sub-clinical mastitis) and experimental group Ⅱ (cows with clinical mastitis). N-acetyl-β-D-glucosaminidase (NAGase) activity of milk samples was detected and the relationship between cow′s mastitis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the promoter of cow lactoferrin gene was explored. The results showed that there were significantly differences in NAGase activity among groups (P<(0.01)) and polymorphism existed in the promoter of cow lactoferrin gene, which suggested that this polymorphism could be related to cow′s mastitis affection and might become a molecular marker of the mastitis.