应用SSR分子标记鉴定超级杂交水稻组合及其纯度

Identification and Purity Test of Super Hybrid Rice with SSR Molecular Markers

应用SSR分子标记技术对超级杂交稻5 个组合(HYS 1/R105、培矮64S/E32、两优培九、88S/0293、J23A/Q611)及其9个亲本进行了鉴定。用144对SSR引物进行筛选,有47对能够在实验材料中显示较好的多态性,其中,RM337与RM154呈现丰富多态性,可鉴别供试组合并分别与其亲本区分开。对于水稻的每一条染色体,各筛选出两条产生多态性的引物,共24对,并提供一组作为鉴定参考的图谱;通过杂种表现为父母本互补带型的特点,找到在杂交稻组合及其亲本间具有多态性的引物,筛选出5对引物分别作为鉴定上述5个超级杂交稻组合的特异引物,进而针对杂交稻不同的纯度问题设计鉴定方法。

Five super hybrid rice combinations and their parental lines were tested by means of SSR analysis. A total of 144 SSR primer pairs distributing on 12 rice chromosomes were used, 47 of them showed polymorphism. Among all these primers, RM337 and RM154 produced polymorphic patterns in four or more of the tested experimental materials respectively, and they could distinguish almost all the rice genotypes tested. Twenty four primer pairs, two on each rice chromosome, were selected to make a reference SSR marker based fingerprinting for the rice lines. For most of primer pairs, F 1 hybrids mainly showed complementary pattern of both parents, which could be very useful to distinguish the F 1 from its parental lines. In addition, five primer pairs were selected as special primer pairs for five hybrid rice combinations respectively. By combining the rapid, simple method on DNA extraction, it is suggested that SSR technique has wide prospective in variety authentication and purity identification.