稻瘟病菌株CH63和TH16杂交组合的遗传图谱构建及无毒基因定位

Construction of Genetic Linkage Map and Location of Avirulence Genes from Cross CH63 and TH16 of Magnaporthe grisea

测定了CH63和TH16杂交后代(F1 代子囊孢子群体)在36个水稻品种上的致病性,依据F1 代子囊孢子群体在不同品种上的无毒性/毒性表型和SSR、SCAR和RAPD等分子标记的多态性位点共分离构建了稻瘟病菌的遗传图谱。图谱共有151个标记位点,7个连锁群,全长1038.4 cM。由杂交后代在不同品种上的无毒性/毒性分离鉴定出CH63菌株持有多个无毒基因,在该遗传图谱中初步定位了Avr2 K59、Avr C105 TTP1、Avr Zh156、Avr Xuan1641、Avr Xuan6392 和Avr C103 TTP 6个无毒基因,分别定位在第1、第4和第7染色体上。

A framework genetic map of Magnaporthe grisea has been constructed using F 1 progeny derived from the cross between M. grisea isolates CH63 and TH16. A genetic map was constructed with 151 markers, which included 126 SSR, 9 SCAR, 1 RAPD markers assigned to seven linkage groups with a total map length of 1038.4 cM. The avirulence genes Avr2 K59, Avr C105TTP1, Avr Zh156, Avr Xuan1641, Avr Xuan6392 and Avr C103TTP were located on this genetic linkage map. Avr2 K59 and Avr C103TTP were located on chromosome 1, Avr C105TTP1 on chromosome 4 and the others on chromosome 7.