水稻不同外植体培养效果及其相关性分析

Effects of Tissue Culture with Different Explants and Their Relationship in Rice

以23个水稻品种(组合)或品系(基因型)为材料,对不同外植体(花药、幼穗、幼胚和成熟胚)进行愈伤组织诱导和绿苗分化,结果表明,4种外植体的培养效果存在基因型差异,不同外植体的培养效果差异明显。花药与幼穗、成熟胚间的愈伤组织诱导率呈显著正相关,幼穗与幼胚的愈伤组织诱导率也呈显著正相关;绿苗分化率之间则不存在相关性;4 种外植体的愈伤组织诱导率和绿苗分化率之间无相关性。

Different explants (anther, young panicle, young embryo and mature embryo) of 23 rice genotypes were used for callus induction and green plantlet differentiation by tissue culture. There existed difference in tissue culture effect with four different explants in genotypes, and significant difference among different explants.Significant positive correlations were found in callus induction frequencies between anther and young panicle and mature embryo, as well as young panicle and young embryo. No correlationships were found among green plantlet differentiation frequencies of different explants, and between callus induction frequency and green plantlet differentiation frequency.