健骨颗粒对去卵巢骨质疏松模型鼠钙调节激素的影响

Effect of Jiangu Granula on Ca^(2+) regulating hormone of ovariectomized rats

目的探讨健骨颗粒对骨质疏松模型鼠三种主要钙调节激素水平的影响。方法切除雌性大鼠卵巢建立绝经后骨质疏松症病理模型,分别喂服健骨颗粒、骨松宝和生理盐水,用放射免疫测定法检测血清甲状旁腺激素(PTH)、1,25二羟维生素D3[1,25(OH)2D3]、降钙素(CT)和雌二醇(E2)水平。结果卵巢切除后随着血清E2水平的下降,1,25(OH)2D3、CT含量明显下降(P<001或P<005),而PTH却显著上升(P<001)。用药后健骨颗粒组血清1,25(OH)2D3、CT水平显著回升(P<001或P<005),而PTH浓度出现下降(P<001)。E2与CT、1,25(OH)2D3呈正相关,而与PTH呈负相关;PTH与1,25(OH)2D3、CT呈负相关,1,25(OH)2D3与CT呈正相关。结论健骨颗粒能通过协调骨质疏松模型鼠血清PTH、1,25(OH)2D3、CT三种钙调节激素浓度,改善因E2下降对骨代谢产生的副作用,这可能是健骨颗粒有效防治骨质疏松症的主要作用机制之一。

Objective:To investigate the effect of Jiangu Granula(JGG) on the concentration of three Ca^2+ regulate hormone of osteoporosis(OP) rats.Methods:SD rats with ovariectomy were randomly divided into three groups:Normal saline group,Gusongbao group,JGG group.The serum concentrations of PTH,CT,1,25-(OH)_2D_3,E_2 were measured by radioimmunoassay(RIA).Results:The levels of 1,25-(OH)_2D_3 and CT in serum were decreased(P<0.01 or P<0.05)at 12 weeks after operation,but PTH were increased.The levels of 1,25-(OH)_2D_3 and CT in the JGG group were increased,but PTH were decreased compared with normal saline group.We found that there was a negative correlation between E_2 and PTH,PTH and 1,25-(OH)_2D_3,CT,but it has a positive correlation between E_2 and CT,1,25-(OH)_2D_3,so as to 1,25-(OH)_2D_3 and CT.Conclusion:JGG can coordinate comprehensively the concentration of PTH,1,25-(OH)_2D_3,CT ,and reduce side effects to bone metabolism caused by the decline of E_2 level.Maybe it is the one of main active mechanisms that JGG can cure or prevent effectively from the postmenopausal osteoporosis.