网孔纳米羟基磷灰石/聚酰胺人工骨修复兔桡骨缺损

Repair of bone defect with porous composite of nano-hydroxyapatite and polymide

目的 探讨新型网孔纳米羟基磷灰石 /聚酰胺 66(n-HA/PA66)复合材料作为骨组织工程支架修复长骨骨缺损的能力及作为人工骨修复替代材料的可行性。 方法 选用新西兰大白兔双侧桡骨制作骨缺损模型,将n-HA/PA66复合材料植入左侧骨缺损处,右侧骨缺损以脱蛋白骨(deproteinelbone, DPB)植入作为实验对照,另做不植入任何材料的骨缺损空白对照,同时设置未行任何处理只供力学测试的正常对照。在 2, 4, 8, 12, 16周各时相点分别进行大体观察、X线照片、组织学切片、扫描电镜观察及力学测试。 结果 n-HA/PA66人工骨组与牛脱蛋白骨(bDPB)组骨缺损均完全修复,而空白对照组骨缺损未见修复;n-HA/PA66与bDPB两组间及两组分别与正常对照组生物力学各项测试指标比较,差异均无统计学意义 (P>0. 05)。 结论 新型骨修复和重建材料n-HA/PA66具有良好的骨传导能力和力学特性以及生物相容性,有望成为骨组织工程中修复骨缺损的理想支架材料。

Objective To evaluate the ability of porous nano-hydroapatite crystals and polyamide composite (n-HA/PA66) in repairing bone defects and discuss the feasibility of n-HA/PA66 as bone substitute material. Methods The animal models of bilateral radius bone defect created by surgery in the New Zealand white rabbits were implanted with n-HA/PA66 on the left bone defect (experimental group) and with deproteined bone (DPB) on the right defects (control group). The bone defects with no implant were used as blank control group. Normal control was set in rabbits that received nothing of treatment. The effect was observed by gross, X-ray, histopathological, transmission electron microscopic and dynamic examinations 2, 4, 6, 8, 12 weeks after operation. In the meantime, the interface of implants and receptor bone was observed by the scanning electron microscope and biomechanics determined 16 weeks after operation. Results The defects of experimental group and experimental control group got repaired perfectly. There was no statistically significant difference between experimental group and control group upon biomechanics (P>0.05). Conclusion n-HA/PA66 has good biocompatibility and osteoconductive ability and may be one of ideal scaffolds in repairing bone defects in bone engineering.