大鼠肺炎链球菌性脑膜炎脑组织明胶酶A、B mRNA的表达及活性分析

The mRNA expressions and activities of gelatinase-A and B in pneumococcal meningitis in rats

目的:研究大鼠肺炎链球菌性脑膜炎脑组织明胶酶A、明胶酶BmRNA的表达及活性的变化。方法:建立幼大鼠脑膜炎模型,用逆转录-聚合酶链反应法测定脑组织明胶酶A、BmRNA表达;用SDS-PAGE明胶酶谱学测定脑脊液中明胶酶A、B的活性。结果:大鼠蛛网膜下腔和软脑膜发生炎症反应,脑组织明胶酶BmRNA表达显著增强(1.04±0.34,P<0.01);脑脊液中明胶酶B活性也显著增高(1554.2±264.5,P<0.01)。而明胶酶AmRNA表达及酶活性无显著改变(P均>0.05)。结论:明胶酶B参与了肺炎链球菌性脑膜炎的病理生理过程。

Objective: To study the mRNA expressions and levels of gelatinase-A and B in pneumococcal meningitis. Methods: Thirty-six wistar rats were infected intracisternally with streptococcus pneumoniae, and their pathological changes were observed. The mRNA expressions of gelatinase-A and B in brain parenchyma were verified by RT-PCR analysis, and gelatinolytic activities of gelatinase-A and B in cerebrial spinal fluid(CSF) were determined by gelatin zymographic analysis. Results: Acute inflammation was observed in subarachnoid space. The mRNA expression and relative gelatinolytic activity of gelatinase-B in pneumococcal group were significantly increased (1.04±0.34;1 554.2±264.5, respectively), but there were no changes in the expression and relative gelatinolytic activity of gelatinase-A (P>0.05). Conclusion: There is an up-regulation of gelatinase-B which may contribute to the pathogenesis of pneumococcal meningitis.