丹参对肝星状细胞胞浆游离钙的影响

Effects of Radix Salviae Miltiorrhizae on Ca^(2+) in Hepatic Stellate Cells

目的 研究丹参药物血清对肝星状细胞 (HSCs)胞浆游离钙 ([Ca2 + ]i)的影响。方法 制备肝纤维化模型鼠。造模毕 ,药物组以每天 2 0 m l/ kg剂量分 2次灌服丹参 ,对照组灌以等量生理盐水。连续给药 6 d后经下腔静脉取血并分离血清。采用盲法 ,用上述 10 %药物血清培养 HSCs2 4 h,再将经药物血清预处理的细胞及对照组细胞负载 Fluo- 3/ AM后使用激光扫描共聚焦显微镜 (L SCM)检测 [Ca2 + ]i。结果 1正常丹参药物血清及肝纤维化药物血清预处理后均可明显减低活化的 HSCs的 [Ca2 + ]i,其荧光强度相对值与病理模型对照组的差异有统计学意义 (P<0 .0 5 ) ;且两者与正常对照组的差异也有统计学意义 (P<0 .0 5 )。 2正常丹参药物血清预处理后加入血管紧张素 (Ang- ) ,[Ca2 + ]i荧光强度变化百分数显著低于病理模型对照组 (P<0 .0 5 ) ;肝纤维化药物血清组荧光强度变化百分数也显著低于病理模型对照组 (P<0 .0 5 )。结论 丹参能抑制肝星状细胞 [Ca2 + ]i的升高 ,可能是其发挥抗肝纤维化作用的重要途径之一。

Objective To investigate the effects of Radix Salviae Miltiorrhizae(RSM) on intracellular free calcium in hepatic stellate cells (HSCs). Methods After the model of hepatic fibrosis was established in SD rats, RSM 〔20 ml/(kg·d)〕 was given via gastrogavage to the rats of treatment groups while the same volume of 0.9% NaCl was given to the rats of control groups twice a day for 6 consecutive days. Then the blood sample was drawn from the inferior vena cava, and the serum was extracted for pharmacological studies. After 24 h incubation with 10% drug serum, HSCs were loaded with Fluo-3/AM, a Ca 2+ marker,and were observed with laser scanning confocal microscopy(LSCM). Results By comparison with controls, both RSM pharmacological serums decreased [Ca 2+ ]i in HSCs significantly in the condition of using Ang Ⅱ or not (P<0.05). Conclusion RSM decreased [Ca 2+ ]i in activated HSCs, which may be one of important ways to block liver fibrosis.