光化学－化学发光法测定蔬菜中的维生素C

Determination of Vitamin C in Vegetables Using Photochemical-Chemiluminescent Method

光化学－化学发光法测定蔬菜中的维生素Ｃ王尊本，郑朱粹，肖利民（化学系现代分析化学研究所）食品中维生素Ｃ的测定可用荧光法、２，４－二硝基苯肼比色法、２，６－二氯酚靛滴定法和磷钼兰比色法等［１］．但这些方法有的操作较烦，有的选择性较差，有的准确度不够好．...

A method of photochemistry-chemiluminescence for the determination of vitamin C in the vegetables is presented. In the acid medium,I￣ turns into I_2on irradiation of ultraviolet light. Part of I_2formed reacts quantitatively with vitamin C and that reniained is made to react with luminol in a PH 13. 0 buffer solution of H_3BO_3-KOH. By measuring the chemilunescent intensity,the quantity of I_2remained could be determined,and thus the content of vitamin C in the sample could be estimated indirectly. The linear relative coefficient of standard curve is more than 0. 99 and the recoveries obtained are between 92. 1% and 104%.