生物功能电极Ⅱ．酶修饰电极反应动力学

Biofunctional ElectrodesⅡ. Reaction Kinetics at Enzyme Modified Electrodes

分别用吸附交联法和共价键合法将葡萄糖氢化酶修饰在破碳电极表面上，并用循环伏安法和旋转圆盘电极技术研究其生物电催化动力学．提出酶修饰电极的界面模型并导出电极上酶促反应的稳态动力学表达式，从而指出由电化学方法测得的固定化酶表观动力学参数的物理意义．讨论了酶修饰电极的电而响应特性与固定化酶的性质、酶电极界面的几何特征、溶液中的传质以及电子传递体在电极基体上的电荷传递动力学等因素的关系．

The enzyme glucose oxidase was modified on glassy carbon electrodes byadsorption-crosslinkage and covalent attachement respectively,and the bioelectrocatalytic ki-netics at the resulting enzyme electrodes was studied with cyclic voltammetry and rotatingdisk electrode technique. A model was proposed for the interfacial region at the enzyme elec-trode,thereby the expressions were derived for the steady kinetics of enzyme catalytic reac-tion and the physical significance of apparent kinetec parameters obtained from electrochemi-cal measurement was illustrated.The current responses of the enzyme electrodes wete anal-ysed as the functions of various factors such as inherent properties of immobilized enzyme,geometric factor of the electrode interfacial region,mass transport in solution and electron-transfer kinetics of mediator.