摘要
从厦门文昌鱼体内提纯了一种酸性磷酸酶,用快速蛋白液相层析法(FPLC)的Su-perose12柱和MonoS柱对其进一步纯化,用聚丙烯酰胺凝胶电泳和FPLC的Superose12柱鉴定为单一纯的酶制剂.它的分子量为52000,经SDS聚丙烯酰胺凝胶电泳鉴定该酶为二聚体.酶的提纯倍数为612.52.
ACPase was isolated from Branchiostoma belcheri(Gray),and purifiedby the additional application of FPLC columns such as Superose 12 column and Mono S col-umn. Only one zone was show by polyacrylamide gel electrophoresis and a single peak onFPLC column for the final product and purified 612. 52 fold Its molecular weight was 52,000and consisted of two identical subunits indicated by SDS polyacrylamide gel electrophoresis.
出处
《厦门大学学报(自然科学版)》
CAS
CSCD
北大核心
1994年第5期681-685,共5页
Journal of Xiamen University:Natural Science
关键词
文昌鱼
酸性磷酸酶
FPLC法
Branchiostoma belcheri,ACPase,FPLC Purificatiom