高血压病患者淋巴细胞端粒酶活性的研究

A Study of Telomerase Activity of Lymphocytes in Patients with Essential Hypertension

目的 探讨高血压病患者外周血淋巴细胞的端粒酶活性。 方法 采用聚合酶链反应 -酶联免疫吸附(PCR -ELISA)方法测定 91例高血压病患者和 43例血压正常者外周血淋巴细胞端粒酶活性。 结果 高血压组外周血淋巴细胞的端粒酶活性 (A值 0 .45± 0 .2 2 )比正常对照组 (A值 0 .2 4± 0 .11)明显增高 (P <0 .0 0 1) ,根据血压水平分级和定量预后的危险因素分层各组间端粒酶活性无显著性差异 (P >0 .0 5 )。 结论 高血压病患者外周血淋巴细胞的端粒酶活性增高 ,这种酶的变化并不随血压水平和预后危险因素的增加而增加。

Objective To investigate the telomerase activity of peripheral blood lymphocytes in patients with essential hypertension. Methods The telomerase activity of peripheral blood lymphocytes in 91 hypertensives and 43 normotensives was measured with Telomerase PCR-ELISA assay. Results The telomerase activity of peripheral blood lymphocytes in hypertensive group was singnificantly increased than that in control group(0.45±0.22 and 0.24±0.11,respectively, P< 0.001). According to level of hypertension and prognostic risk factors, telomerase activity of peripheral blood lymphocytes was upregulated to the same level in every group(P>0.05). Conclusion The telomerase activity of peripheral blood lymphocytes in hypertensive group was increased. It was found that the increased telomerase activity were not correlated with the severity of hypertension and prognostic risk factors.