一株新的卵巢癌细胞系的建立及其生物学特性分析

A Biological Characteristics Study and Establishment of a New Human Ovarian Carcinoma Cell Lines

目的 从人卵巢癌组织建立一株卵巢癌细胞系 ,为卵巢癌发病机制研究和治疗药物筛选提供可靠的材料。 方法 取手术切除的卵巢癌组织 ,进行组织块培养法 ,待长满 90 %汇合面后消化传代培养 ,并进行形态学、生长动力学及致瘤性等生物学特性分析。 结果 该卵巢癌细胞系已在体外培养生存 1年以上 ,传 80余代 ,命名为湖南卵巢癌细胞系 1号 (HOC1) ,其生物学特性显示 :这些细胞系体外生长的倍增时间为 47.2h ;软琼脂集落形成率为 18.5 % ;接种至裸鼠后具 10 0 %致瘤性 ;染色体核型分析为非整倍体 ,众数为 74～ 92条 ;透射电镜下可观察到卵巢癌细胞表面含有丰富的微绒毛 ,胞浆内含有丰富的核糖体。 结论 该卵巢癌细胞系经体外长期培养后已形成永生化细胞系 ,并具有明显的恶性表型特征。

Objective To establish an ovarian carcinoma cell line from human ovarian carcinoma surgical specimen,which provide a reliable data for studying the pathogenic mechanism and screening of medicine of ovarian carcinoma. Methods The tissues from human ovarian carcinoma biopsies were cultured in RPMI1640 media. After growing to 90% confluence, the cells were indigested for subculture, whose characteristics, including morphology, growth kinetic and tumorigenecity in nude mice, were studied. Results This cell line has been passaged more than 80 times within 1 year which called HOC1(Human Ovarian Carcinoma cell line). The Biological Characteristics of HOC1 demonstrated: The population doubling time calculated in the log phase of growth was 47.2 h. The cloning efficiency in soft agar averaged 18.5%. The ovarian car cinoma cells has high tumorgencity in nude mice. Karyo-type analysis showed aneuploidy with a modal chromosomal number 74～92. Under Electronmicroscopy, there was rich microvilli on cell surface and abundant ribosomes in cytoplasm. Conclusion The ovarian carcinoma cells have immortalized after being cultured in vitro and had specific malignant characteristics of ovarian carcinoma.