NGF与CNTF促进周围神经轴浆运输功能恢复的比较研究

Comparative study on the functional recover of axoplasmic transport of regenerative nerve promoted by nerve growth factor or ciliary neurotrophic factor

目的: 比较神经生长因子 (nervegrowthfator, NGF) 与睫状神经营养因子 (cili aryneurotrophicfactor, CNTF) 对周围神经轴浆运输功能恢复的促进作用。方法: 利用自行研制的梭形双通道桥接管桥接 32只SD大鼠坐骨神经长 10mm缺损。将动物随机分为 2组。A组: 医用几丁糖凝胶组; B组: 医用几丁糖凝胶+NGF和医用几丁糖凝胶 +CNTF。术后 12、16周对再生神经行辣根过氧化物酶和核黄逆行示踪, 并取再生神经行Holmes银染。结果: 术后 12、16周, Holmes银染观察到A组两支管内再生纤维无明显差异, B组CNTF侧再生纤维较NGF侧再生神经外膜薄, 排列整齐, 粗细均匀; 辣根过氧化物酶和核黄显示: 与NGF侧相比,CNTF侧再生神经示踪后, 脊髓内被标记的阳性神经元数目多, 分布稠密。结论: CNTF对周围神经再生纤维形态结构和轴浆运输功能的恢复均优于NGF。

Objective:To compare the effects of recover of axoplasmic transport of regenerative nerve promoted by nerve growth factor(NGF) or ciliary neurotrophic factor(CNTF).Method:Thirty-two Sprague-Dawley(SD) rats with 10mm defect of sciatic nerve were randomly divided into two groups which had been bridged with the new designed double channel nerve conduit of fusiform shape. Each groups contained twelve animals, in the first group, 200μl of chitin for medical use was injected into the conduit, in the secondgroup, the two branches of the conduit contained 100μl of the chitin and 5 μl NGF or CNTF.At the 12^(th) and 16^(th) week after operation,the authors first evaluated the axoplasmic transport of regenerative nerve in the two branch channels with Horseradish peroxidase(HRP) and Nuclear yellow(NY),and observed quality and quantity of the regenerative fibres with Holmes staining.Result:At the 12^(th) and 16^(th) week after operation,there were not significant differences of the regenerative nerve fibres between two channels in the first groups, but in the second group, there were more and well-arranged regenerative fibres in branch contained the CNTF, when the tracing substance of (HRP) and NY were injected to the regenerative nerve in the branch contained CNTF, the positive neurons in the spinal cord was much more and densely distributed.Conclusion:The effects of CNTF on functional recover of axoplasmic transport of regenerative fibres is superior to that of NGF