小鼠胚胎发育中期肺细胞增殖与凋亡的研究

Cell proliferation apoptosis and expression of related genes in developing lung of mouse

目的探讨小鼠胚胎中期肺发育过程细胞增殖与凋亡的变化规律及相关基因p21、ki-ras在肺发育中的表达意义。方法采用免疫组织化学及切口末端标记法(TUNEL),观察小鼠肺发育过程中的细胞增殖与凋亡。用原位杂交的方法检测p21、ki-ras在胚胎肺发育中期的表达。结果肺组织在第12天细胞增殖的表达较强,而到第13天、第14天的细胞增殖趋于稳定。p21、ki-ras基因的表达主要在肺上皮细胞,随胎龄增长而表达颜色加深。结论胚胎发育中期细胞增殖与凋亡相伴存在。p21、ki-ras基因在胚胎肺发育中期有表达,说明这两个细胞周期调控基因在胚胎肺发育中期起一定的作用。

To investigate the dynamic trends of cell proliferation, apoptosis and the expression significance of involved genes as p21 and ki-ras in developing lung of mouse. Lung tissues were taken from different developmental phases of kunming mouse. Immunohistochemistry and TUNEL techniques were adopted to observe the variation of cell proliferation and apoptosis and the expression characteristics of p21 and ki-ras was detected by in situ hybridization. We discover that cell proliferation increased greatly in the 12th day while from the 13th to the 14th the proliferation maintained stead raise. With the development of embryos , the expression of p21 and ki-ras have a darker colour in the epidermic cell of lung. [Conclusions] Cell proliferation is accompanied by apoptosis during the development of mouse lung.The dynamic changes of both ratios might be correlated to the different structures appearing in different developmental process. It suggests that p21 and ki-ras may play a role in embryo development.