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异种卵泡液对牛卵母细胞体外成熟率的影响 被引量:2

Effects of Follicular of Different Species on In Vitro Maturtion Rate of Bovine Oocytes
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摘要 本研究探讨了异种卵泡液对牛卵母细胞体外成熟率的影响。结果表明,在培养基中添加适当浓度的山羊、绵羊、骆驼卵泡液代替血清,牛卵母细胞的成熟率(67.9%、68.5%、65.3%)与血清对照组(72.0%)无显著差异(P>0.05);在添加山羊、绵羊、骆驼卵泡液的基础上补充激素,卵母细胞的成熟率(70.8%、72.9%、69.7%)也未见明显提高(P>0.05)。 This study was undertaken to examine the effect of follicular of different species on in vitro maturation rate of bovine oocytes.The result showed:Goat sheep and camal follicular of proper concentration were added to culture media in place of serum,the in vitro maturation rate of oocyte(67.9%、68.5%、65.3%)was not significantly different in comparison with the oocyte matured in culture media with serum(P>0.05);When folliclar and hormone were added in culture media,the in vitro maturation rate of oocyte(70.8%、(72.9%)、69.7%)did not increase significantly(P>0.05).
出处 《草食家畜》 2005年第1期34-35,共2页 Grass-Feeding Livestock
关键词 牛卵母细胞 体外成熟 绵羊 山羊 骆驼 卵泡液 成熟率 异种 血清 对照组 follicular fluid oocyte mature rate
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参考文献5

  • 1崔亚利,桑润滋.牛卵母细胞体外成熟及体外受精的研究[J].河北农业大学学报,1999,22(1):67-70. 被引量:25
  • 2Sirard M A,Lambert R D.In vitro inhibition of oocyte nuclear maturation in the bovine[J].Biol-Reprod,1988,(39):229~230.
  • 3Fukui Y,One H.Effects of sera homones and granulosa cells added to culture medium for in vitro maturation,fertilization,cleavage and development of bovine oocyte[J].J Repred Fert,1989,(86):501~505.
  • 4朱玉林.牛卵泡液对牛卵母细胞体外成熟的抑制作用[J].草食家畜,1995(4):34-37. 被引量:4
  • 5Naito K,Fukuda Y,Toyoda Y.Effect of follicular fluid on male pronuleus formation in porcine oocyte matured in vitro[J].Gamete Research,1998,21:289~295.

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