小鼠实验性肿瘤NO含量与微血管密度的相关性研究

Relationship between nitric oxide content and mcirovessel density in experimental tumor in mice

目的:探讨小鼠实验性肿瘤NO含量与微血管密度的关系。方法:以肝癌细胞(H22)接种小鼠后肢肌群建立肿瘤模型。实验小鼠随机分为两组:(1)肿瘤组(Tu,n=88);(2)对照组(Tc,n=88)。前一组接种活的肝癌细胞,后一组接种高温灭活的肝癌细胞。在接种后的不同时间,检测接种部位 NO含量和微血管密度。结果:随着肿瘤生长,肿瘤组织中NO含量及微血管密度逐渐增加,与对照组比较有显著差异(P< 0.01), NO 含量与微血管密度呈显著正相关( r=0 .9145)。结论:提示NO可能有促进肿瘤微血管生长作用,从而促进肿瘤生长。

Objective: To study the relationship between ni tric oxide(NO) content and mcirovessel density in experimental tumor. Methods: The hepatic cancer cells(H22) were vaccinated in the muscles in hind limbs of mice to establish the experimental model of tumor. The mice we re divided randomly into two groups, i.e. tumor group(Tu, n=88) and control grou p(Tc, n=88). The Tu group was vaccinated with the live hepatic cancer cells and Tc group was vaccinated with the death hepatic cancer cells killed by high tempe rature. At the different time after these mice were vaccinated, the NO content a nd microvessel density around the injected sites were examined. Resul ts: The NO content and microvessel density increased continuously along with tumor growth in Tu group and were significantly different from those in th e control group since the third day(p<0.05). The NO content was correlated posit ively with the microvessel density(r=0.9145). Conclusion: It is suggested that NO may contribute to the growth of tumor by means of stimulat ing the growth of microvessels in tumors.