SR-BI基因Gly4Ser多态性对2型糖尿病血脂的影响

The Influence of Gly4Ser Variant of the Scavenger Receptor Class B Type I Gene Exon 1 Polymorphism on the Serum Lipid of Type 2 Diabetes Mellitus

目的 探讨B族I型清道夫受体 (thescavengerreceptorclassBtypeI,SR BI)基因外显子 1的Gly4Ser多态性与 2型糖尿病血脂的关系。方法 用聚合酶链反应—限制性片段长度多态性分析方法 ,检测 15 0例 2型糖尿病患者 (糖尿病组 )和 12 0例糖耐量正常者 (对照组 )SR BI基因外显子 1Gly4Ser变异的基因型 ;全自动生化分析仪检测空腹血糖 (FBG)、甘油三酯 (TG)、总胆固醇 (TC)、高密度脂蛋白胆固醇 (HDL C)、低密度脂蛋白胆固醇 (LDL C)、脂蛋白A I(apoA I)和脂蛋白B(apoB)等。结果 ①糖尿病组的等位基因频率与对照组相比 ,差异无统计学意义 (P >0 .0 5 ) ;②SR BI基因外显子 1Gly4Ser变异对FBG、TG、TC、apoA I、apoB等均无影响 (P >0 .0 5 ) ;③两组中携带Ser等位基因的个体与Gly/Gly型相比 ,均有着较低LDL C浓度 ,且差异具有统计学意义 (P <0 .0 5 ) ;④在糖尿病组 ,携带Ser等位基因的患者有着较低HDL C浓度 (P <0 .0 5 )。结论 在糖尿病状态下 ,SR BI基因外显子 1Gly4Ser变异可能更容易发挥遗传影响 ,加重 2型糖尿病患者的脂质紊乱。

Objective To investigate the association of scavenger receptor class B type I gene Gly4Ser variant with the serum lipid of type 2 diabetes mellitus.Methods The genotypes of exon 1 variant in SR-BI gene were determined by polymerase chain reaction -restriction fragment length polymorphisms assay in 150 patients with type 2 diabetes mellitus and 120 individuals with normal glucose tolerance(NGT).Fasting plasma glucose(FBG),triglycerides(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C), apolipoprotein A-I(apoA-I)and apolipoproteinB(apoB) were detected by automatic biochemistry analyzer. Results ① The allele frequency of type 2 diabetes mellitus group was not significantly different from that of NGT (P>0.05). ② FBG,TG,TC,apoA-I and apoB were not significantly influenced by SR-BI Gly4Ser variant (P>0.05). ③ Compared with Gly/Gly genotype, subjects with Ser genotype had significantly lower LDL-C concentration in both groups (P<0.05). ④ The diabetic subjects with allele Ser had significantly lower HDL-C concentration in type 2 diabetes mellitus group(P<0.05).Conclusion SR-BI gene exon 1 Gly4Ser variant could promote the development of dyslipidemia and especially in T2DM.