薄层扫描法测定苦参中的苦参碱和氧化苦参碱的含量

Quantutative Determination of Matrine and oxymatrinein Ku shen by TLC-scanning

目的:测定苦参中苦参碱、氧化苦参碱的含量。方法:薄层扫描法。结果:苦参碱的含量为2 .118mg/g ,S =0 .0 5 7,RSD =2 .6 9% ;氧化苦参碱的含量为3.894mg/g ,S =0 .0 4 4 ,RSD =1.13%。结论:苦参碱的含量比氧化苦参碱的含量低;薄层扫描法结果准确性好、重现性好。

objective: To establish a quantity determination of matrine and oxymatrine in Ku shen. Method: To establish quantity by wavelength and to use TLC-scanning. Result: Quantity of matrine is 2.118mg/g in Ku shen. S=0.057,RSD=2.69%. Quantity of oxymatrine is 3.894mg/g in Ku shen. S=0.044,RSD=1.13%. Summury: we determined quantity of matrine and oxymatrine by thin layer chromatography scanning. So,in Kushen quantity of matrine is littier than quantity of matrine in oxymatrition Ku shen. Result's optimal quality of TLC-scanning method is good,a new discovered quality is good,selected color's secretion is constancy and has long time quality.