丝素膜表面磺酸化研究

SURFACE SULFONATION MODIFICATION OF SILK FIBROIN MEMBRANE

从蚕丝中提取丝素蛋白制备成纯丝素膜及PEGO丝素膜,首次采用硫酸处理,在丝素膜材料表面引入了磺酸基团;采用电子能谱和全反射红外光谱分析材料的表面性质,表明硫酸处理丝素膜,可以使PEGO丝素膜表面带上磺酸基团,以共价键的结合方式牢固地固定在表面。这将大大提高丝素膜的抗凝血性,防止细胞在丝素膜上的粘附。X射线衍射表明,丝素膜在硫酸处理后,其结晶度增加,特别是silk 的结晶,但是,经硫酸处理后,丝素膜的断裂伸长率与强度有所下降。

Silk fibroin membrane and PEGO silk fibroin membrane were treated with sulfuric acid to introduce functional groups on the surface of membranes. EDS and ATR-FT-IR were used to analyze the surface chemical elements. The structure of modified membranes was characterized by X-ray diffraction. It shows that sulfonic group is covalently grafted on the surface of the PEGO silk fibroin membrane after treatments, and it contains silk Ⅰ crystal. After treated with sulfuric acid, the membranes have worse tensile strength and lower elongation at break.