流式DNA倍体分析在卵巢肿瘤腹水中的应用

The application of flow cytometry analysis in ascites of ovarian tumors.

对８１例卵巢肿瘤的腹水或腹腔冲洗液进行流式倍体分析。５４例恶性肿瘤中发现４５例为异倍体，与术后病理诊断附合率为８３．３３％。２７例良性肿瘤仅３例为异倍体，与病理附合率为８８．８８％。对４７例卵巢癌同时进行细胞学检查，阳性率仅为２９．６２％，显著低于流式分析结果。腹水的流式ＤＮＡ倍体分析为识别良，恶性腹水开辟了一条新的诊断途径。

Flow cytometry was used in DNA ploidy analysis of tumor cells in ascites or washing fluid of eighty one cases with ovarian tumors. Fourty five were found to be aneuploidy n fifty four cases with malignant tumors, the corresponding rate with pathologic diagnosis after operation was 33. 3%.Among twenty seven benign tumors only three were aneuploidy the corre ponding rate with pathologic diagnosis was 38.88% . Cellular examination was made at the same time for fourty seven malignant ones,its positive rate was only 29.62% which was obviously lower than the result of flow cytometry. DNA ploidy analysis with flow cytometry opened up a new diagnostic way for distinguishing benign and malignant ascites.