益肺健脾方对慢性阻塞性肺疾病患者痰液炎症细胞计数和IL-8、TNF-α水平的影响

Effect of Yifei Jianpi Recipe on Inflammatory Cells, Levels of Interleukin-8 and Tumor Necrosis Factor-α inSputum from Patients with Chronic Obstructive Pulmonary Disease

目的 探讨益肺健脾方对慢性阻塞性肺疾病(COPD)的作用及其机制。方法 将40例稳定期COPD患者随机分为治疗组和对照组,观察其治疗前后痰液炎症细胞计数与分类、白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)、1秒钟用力呼气容积占预计值百分比(FEV1占预计值％)、1秒钟用力呼气容积／用力肺活量(FEV1／FVC)等指标变化,并与20名健康人作比较。结果 COPD患者治疗前后各指标与健康人比较差异均有显著性(P<0.01)。治疗后治疗组痰液中性粒细胞(PMN)、IL-8和TNF-α水平较治疗前显著降低(P<0.01),非中性粒细胞(nPMN)、FEV1占预计值％和FEV1／FVC显著升高(P<0.01);对照组仅FEV1占预计值％和FEV1／FVC明显升高(P<0.05,P<0.01)。治疗组降低PMN、IL-8和TNF-α水平,升高nPMN、FEV1占预计值％和FEV1／FVC的作用显著优于对照组(P<0.01)。结论 益肺健脾方可减轻COPD患者气道炎症反应而发挥治疗作用。

To explore the effect and the mechanism of Yifei Jianpi Recipe (YFJPR) on patients with chronic obstructive pulmonary disease (COPD). Methods Forty patients with COPD in stable phase were randomly divided into two groups, the treated group and the control group. Indexes including the total and differential count of inflammatory cell in sputum, levels of interleukin-8 (IL-8) and tumor necrosis factor-α(TNF-α) , as well as the percentage of forced expiratory volume in one second in its predicted value (FEV1 % ) and ratio of FEV1/ forced vital capacity (FVC) in patients were measured before and after treatment, and compared with those in 20 healthy subjects. Results All the indexes measured in patients before and after treatment were significantly different from those in healthy subjects (P<0.01). Differential count of polymorphonuclear neu trophil (PMN) and levels of IL-8 and TNF-α in sputum in the treated group significantly decreased after treatment (P<0 .01) , while the non-PMN differential count and levels of FEV1 % and FEV1/FVC significantly increased (P<0.01). But in the control group, changes only showed in increasing of FEV1 % and FEV1/FVC (P<0.05 or P<0.01). And the effects in the treated group were better than those in the control group (P< 0.01). Conclusion YFJPR can play a therapeutic role on patients with COPD by way of reducing the airway inflammatory reaction.