摘要
为了提供市场所需的大量种苗,适应云南烤烟(Nicotianatabacum)规模化生产的需要,以田间烤烟的叶片、茎段、培养瓶内种子萌发的叶片和茎段为外植体进行组织培养实验。实验比较了5种培养基,筛选出烤烟的最佳愈伤组织诱导和愈伤组织不定芽分化培养基为MS+6-BA2mgL-1+NAA.0.2mgL-1+Sugar30gL-1+Agar6gL-1;生根培养基为MS+Sugar30gL-1+Agar6gL-1,生根率为100%;.....用营养土移栽后,存活率为100%。
Using stems with buds as explants, we studied the optimal tissue culture conditions for the rapid propagation of Nicotiana tobacco‘K326’. MS medium with 2 mg L-1 6-BA, 0.2 mg L-1 . . NAA, 30 g L-1 sugar and 6 g L-1 agar was suitable for callus induction and proliferation of bud . . clusters. The rate of rooting reached 100% on MS medium with sugar and agar, and the survival rate of the plants reached 100% after transplanting to soil.
出处
《植物学通报》
CAS
CSCD
北大核心
2005年第1期50-53,共4页
Chinese Bulletin of Botany
基金
云南玉溪红塔烟草(集团)有限责任公司资助项目(384)。
