中国玉米新品种标准DNA指纹库构建研究的几点思考

Review of Research into Establishing a DNA Fingerprinting Database of New Chinese Maize Cultivars

概述了中国玉米新品种DNA指纹库构建研究中的五个主要问题,即选用哪些品种材料、选用什么标记方法、SSR标记存在哪些问题、选用哪些SSR引物及如何提高建库效率等,并提出了相应的解决方案,对其应用前景及主要应用领域进行了探讨。

We summarize five main problems in establishing a DNA fingerprinting database of new Chinese maize cultivars: choice of maize varieties, choice of molecular techniques, problems of simple sequence repeat (SSR) techniques, choice of SSR markers and efficiency in techniques. We present possible solutions and discuss the prospect and main applications for the database.