生态免疫肠内营养保护肠屏障功能的研究

Effect of ecoimmunonutrition on gut barrier function in rats with systemic inflammatory response syndrome

目的探讨生态免疫肠内营养对全身炎症反应综合征(SIRS)大鼠肠屏障功能的保护作用。方法通过尾静脉注射脂多糖(LPS)建立SIRS大鼠模型,60只雄性SD大鼠随机分为4组(标准营养组、免疫增强组、生态营养组、生态免疫组),分别给予不同构成的肠内营养剂7d,观察各组大鼠血浆D-乳酸、二胺氧化酶(DAO)以及尿乳果糖/甘露醇(L/M)比值的动态变化。结果治疗7d后,生态营养组大鼠血浆D-乳酸水平明显低于标准营养组和免疫增强组(P<0.05),但这3组均高于生态免疫组3倍或以上(P<0.01)。血浆DAO水平标准营养组显著高于其他3组(P<0.01)。注射LPS后第1天起,各组大鼠尿液L/M比值均有大幅升高,至第6天回落到注射前水平。第1天时生态免疫组尿L/M比值低于其他3组(P<0.05),第3天时标准营养组尿L/M比值显著高于另外3组(P<0.01),至第6天各组已无明显差异。结论联合应用免疫增强营养素和生态制剂的生态免疫肠内营养能更有效地保护肠屏障功能。

Objective To explore the effect of ecoimmunonutrition on gut barrier function in rats with systemic inflammatory response syndrome (SIRS). Methods Rat models of SIRS were established by injecting lipo polysacchoride(LPS) via the tail vein. Sixty male Sprague Dawley rats were randomly divided into 4 groups. One of the four enteral nutrition formulas (standard EN,immunonutrition,econutrition,ecoimmunonutrition) was given to each group respectively for 7 days. Serum D lactate,diamine oxidase (DAO),ratio of urinary lactulose to mannitol (L/M) were measured on day 1 before LPS injection (D-1) and day 1,3,6 after LPS injection (D+1,D+3,D+6). Results After 7 days of treatment,the level of serum D lactate in econutrition group was significantly lower than those in standard EN or immunonutrition group (P< 0.05),and the level of serum D lactate in all above three groups were significantly higher compared with ecoimmunonutrition group(P< 0.01). The level of serum DAO in standard EN group was higher than those in other three groups(P< 0.01). The L/M was increased in all the groups on D+1,D+3. It fell back to the basic level on D+6 in all the groups.The L/M in immunonutrition group was lower than those in other groups on D+1(P< 0.05),and it was higher in standard EN group than those in other groups on D+3(P< 0.01). Conclusion Ecoimmunonutrition,a enteral nutrition composed of immune enhanced nutrient and ecologics,may protect the gut barrier function more effectively in rats with SIRS.