大鼠外伤性PVR视网膜前膜形成中TERT、PCNA和Bcl-2的变化(英文)

PCNA, Bcl-2 and TERT expression in epiretinal membrane of rat traumatic proliferative vitreoretinopathy

目的:探讨与细胞增殖相关的端粒酶逆转录酶TERT、增殖细胞核抗原(PCNA)和凋亡抑制蛋白Bcl-2在外伤性PVR视网膜前膜形成中的动态变化及其相关性。方法:S-P法对外伤性PVR大鼠模型视网膜前膜做TERT、PCNA、Bcl-2免疫组织化学染色和HE染色,染色结果行图像分析。结果:伤后PCNA蛋白表达阳性细胞率和平均光密度值出现先升高后降低的动态变化,14d组阳性率最高,与7d组和28d组比较有显著性差异。伤后TERT蛋白和Bcl-2表达阳性细胞率和平均光密度值出现先升高后稍降低的动态变化,14d组和21d组阳性率最高,与7d组比较有显著性差异,TERT、PCNA和Bcl-2蛋白表达之间有显著相关性(P ≤0.01)。结论:TERT、Bcl-2参与外伤性PVR视网膜前膜增殖细胞的生长调控,与细胞增殖的动态变化呈高度相关性。

AIM: To explore the dynamic expression and correlation among telomerase catalytic subunit (TERT), proliferating cell nuclear antigen(PCNA)and antiapoptosis protein Bcl-2 which relate to cell proliferation in epiretinal membrane of rat traumatic proliferative vitreoretinopathy (PVR).· METHODS: S-P technique was applied for immunohistochemical staining of epiretinal membrane of traumatic PVR with TERT, PCNA and Bcl-2 antibody. HE staining was also carried out. The staining results were analyzed with image analysis system.· RESULTS: The positive rate and average A of PCNA protein were upregulated at first and then down-regulated, with the peak value in 14d Group, which was significantly different from those in 7d Group and 28d Group. The positive rate and average A of TERT and Bcl-2 were also upregulated at first and then down-regulated, with the peak value in 14d Group and 21d Group, which were significantly different from those in 7d Group. There was significant correlation among PCNA, Bcl-2 and TERT protein expression (P ≤0.01).· CONCLUSION: TERT and Bcl-2 take part in the regulation of proliferative cells in epiretinal membrane of traumatic proliferative PVR, with high correlation with the dynamic changes of cell proliferation.·