应用激光房水细胞仪定量研究PVR模型中PDTC对房水闪光的抑制作用(英文)

A quantitative research on effects of pyrrolidine dithiocarbamate on the aqueous flare in a PVR model with LFCM

目的:应用激光房水细胞仪(LFCM)定量分析PVR动物模型中的炎症反应及PDTC对房水闪光的影响。方法:青紫蓝家兔20只随机等分成2组,在视网膜上制造裂孔后,向第1组所有家兔的右眼(A1组)及第2组所有家兔的左眼(A2组)玻璃体腔注射PDTC0.1mL,向第2组所有家兔的右眼(B1)玻璃体腔注射BSS0.1mL。1h后向实验眼A1组注射BSS0.1mL,向实验眼A2组及实验眼B1组玻璃体腔注射5000UIL-1β。分别于术前及第二次注射后4,24h,1,2及4wk进行临床观察及LFCM检查。病理学及免疫组织化学检查分别在术后4,24h,1,2及4wk进行。结果:在术后24h～2wkPDTC能够明显抑制PVR动物模型中的炎症反应。虽然在术后2wk时应用裂隙灯显微镜观察各组炎症反应已完全消退,但应用LFCM发现实验眼B1组炎症反应仍比另2组明显。病理学及免疫组织化学检查表明IL-1β能够活化NF-κB,PDTC能够抑制其活化而没有明显视网膜毒性作用。结论:在玻璃体腔注射IL-1β诱导的PVR动物模型中有炎症反应的参与,PDTC可以明显抑制此炎症反应。LFCM提供了一种新的,灵敏的,客观和非侵入式的方法对PVR动物模型中的炎症反应进行定量分析。

AIM: To study the inhibitory effect of pyrrolidine dithiocarbamate (PDTC) on the inflammatory reaction in an experimental proliferative vitreoretinopathy (PVR) model with laser flare cell meter (LFCM).· METHODS: A total of 20 pigmented rabbits were divided into two groups randomly, with 10 rabbits in each group. After the creation of retinal holes, 0.1mL PDTC was injected intravitreally into the right eyes of Group 1 (A1) and the left eyes of Group 1 (A2), and 0.1mL balanced saline solution (BSS) into the right eyes of Group 2 (B1). One hour later, 0.1mL BSS into the eyes of A1, and 5000U IL-1β in 0.1mL BSS was injected intravreally into the eyes of A2 and B1. Clinical evaluation and LFCM examination were performed before retinal injury (P0) and at 4h, 24h, 1, 2 and 4wk after the second injection (P4h, P24h, P1wk, P2wk and P4wk). Histopathologic and immunohistochemical examination were also performed at these time points.· RESULTS: PDTC could inhibit the inflammatory reaction obviously from P24h to P2wk. The eyes of A1 and A2 recovered earlier than those of B1. Although inflammatory reaction in the 3 groups resolved completely by the end of P2wk measured with the slit-lamp microscope, the eyes of the B1 still showed obvious aqueous flare judged by the LFCM compared with those of A1 and A2. Histopathologic and immunohistochemical examination showed that nuclear factor-κB (NF-κB) was activated by IL-1β and the PDTC had inhibitory effect on it without obvious toxicity to retina.· CONCLUSION: Inflammatory reaction involves in the rabbit model of PVR induced by injecting intravitreally IL-1β and the PDTC can relieve it significantly. The LFCM provides a new, sensitive, objective and noninvasive method to quantify the inflammatory reaction in the PVR model.·