补肾清热毒方对cGVHD狼疮小鼠肾组织细胞凋亡的调节作用

Regulatory Action of BushenqingreFang on Apoptosis of Nephridial Tissue in Mice with Lupus Nephritis Caused by cGVHD

目的: 探讨补肾清热毒方 (简称补清方) 对慢性移植物抗宿主病 (chronicgraft versus hostdisease, cGVHD) 狼疮样小鼠模型肾组织细胞Fas/FasL (Fasligand) 的调节作用。方法: 采用原位末端标记法 (TUNEL) 染色观察肾组织细胞凋亡; 免疫组化、蛋白印记 (westernblot) 和逆转录聚合酶链反应 (RT PCR) 技术检测Fas、FasL基因转录及蛋白表达情况。结果: 补清方可增加肾组织TUNEL阳性积分, 上调Fas、FasLmRNA和蛋白水平。结论: 补清方治疗狼疮性肾炎 (lupusnephritis, LN) 的作用可能是通过上调Fas、FasL, 促进细胞凋亡实现的。

Objective: To study the regulatory action of BushenqingreFang on Fas/FasL in the cells of nephridial tissue in mice with lupus nephritis (LN) caused by chronic graft-versus-host disease (cGVHD). Methods: To observe the apoptosis in nephridial tissue by TUNEL and to measure the Fas/FasL genetic transcription and their protein expression by immunohistochemistry, western blot and RT-PCR techniques. Results: BushenqingreFang increased the positive score of TUNEL in nephridial tissue and up regulated mRNA expression and protein levels of Fas and FasL. Conclusion: The action of BushenqingreFang on LN may be conducted by its up regulation of Fas and FasL and its promotion of apoptosis.