桑枝多糖分离纯化及其免疫作用的初步研究

The Preliminary Studies on Isolation and Immunity of Ramulus Mori Polysaccharide

采用水提醇沉法从中药材桑枝(Ramulusmori)中分离纯化多糖,并建立桑枝多糖的含量测定方法;采用碳粒廓清法和二硝基氟苯诱导小鼠迟发型变态反应试验法,观察桑枝多糖对地塞米松所致免疫低下模型小鼠免疫功能的影响。结果显示,用改良的苯酚-硫酸法测得桑枝中多糖含量为5.5%,提取物桑枝多糖(RMPS)中的含量为56.6%。多次水溶醇沉后,桑枝多糖含量可达70%以上;桑枝多糖可显著提高免疫低下小鼠的吞噬指数α,增强网状内皮细胞的吞噬功能和小鼠迟发型变态反应能力,增强T细胞活性。

Polysaccharide was isolated and purified from Chinese medicinal herb Ramulus mori (Sangzhi), and determination method for Ramulus mori polysaccharide (RMPS) was developed in this study. Carbon clearance test and dinitrylfluorobenzene-induced delayed type hypersensitivity test (DTH) were used to evaluate effects of RMPS on immune responses of mice treated with dexamethasone. The results indicated that using modified phenol-sulfuric acid method, the content of polysaccharide was 5.5% in Ramulus mori, and 56.6% in RMPS, which exceeded 70% in RMPS after purifying for a few times. The RMPS can improved phagocytic index α, strengthened phagocytosing function of reticulate endothelial cell and the intensity of delayed type hypersensitivity (DTH) reaction, and enhanced the activity of T cells of the mice treated with dexamethasone, significantly.