变形链球菌葡聚糖结合蛋白B功能区片段的分子克隆及表达

Cloning of the functional domains of streptococcus mutans glucan-binding protein B and it's expressiong in E.coli

目的 :克隆变形链球菌葡聚糖结合蛋白B(GbpB)功能区的基因片段 ,并在大肠杆菌中表达。方法 :通过PCR技术克隆GbpB功能区的基因片段并通过蛋白重组融合表达技术使其在大肠杆菌中表达。 结果 :成功克隆了GbpB功能区的基因片段 ,并在大肠杆菌中得到其融合蛋白的表达。 结论 :利用分子生物学技术能够成功克隆GbpB功能区基因并获得其融合蛋白的表达 。

Objective:To clone the functional domains of Streptococcus mutans Glucan-Binding Protein B and express it's fusion protein in Escherichia coli.Method:PCR(polymerase chain reaction) and construction of fusion protein technique.Result:the functional domains of Glucan-Binding Protein B was cloned correctely and it's fusion protein was expressed in Ecoli.Conclusion:PCR(polymerase chain reaction) and construction of fusion protein technique are effective methods to get the aimed genes and it's fusion protein.This experiment has provided a base to the further research.