摘要
目的 探讨武汉地区结核病的分子流行病学规律。方法 采用DNA随机扩增多态性(RAPD)指纹分型法,对 武汉市129例肺结核患者的结核分枝杆菌进行基因指纹分型。结果 129株中以Ⅰ型菌株(46株,35.7%)、Ⅱ型菌株(32 株,24.8%)、Ⅲ型菌株(26株,20.2%)为主。初治与复治者的RAPD指纹类型的分布有显著性差异(P<0.05)。所检菌株耐 药与否,在RAPD指纹类型中分布也有显著性差异(P<0.05)。耐药菌株中主要以耐链霉素为主(81.2%)。结论 RAPD指 纹分型是一种较好的分型方法。武汉地区结核病主要由Ⅰ、Ⅱ、Ⅲ型菌株引起,但耐药病例主要由Ⅳ、Ⅴ、Ⅵ型菌株引起。
Objective Epidemiological studies on Mycobacterium tuberculosis in Wuhan.Methods The fingerprinting method by RAPD to analyze the 129 sputum specimens of lunger in Wuhan.Results Six types of DNA fingerprints were found and the main types were type Ⅰ( 35.7% ),type Ⅱ( 24.8% ),type Ⅲ( 20.2% ). In those main type groups, the distribution of those types in the first treated patients and the retreated patients was distinct different, and the rate of drug-resistance was also statistically different. The main drug-resistant strain was streptomycin-resistant. Conclusion The RAPD fingerprinting was a rapid, precise method to type M.tuberculosis strains. The prevalence of tuberculosis was primarily due to the transmission of typeⅠ, Ⅱ and Ⅲ srains in Wuhan, but the drug-resistant strains were caused by others.
出处
《华中医学杂志》
2005年第1期57-58,60,共3页
Central China Medical Journal
