NF-κB“decoy”寡核苷酸对LPS诱导巨噬细胞IL-10表达的影响

Effects of NF-κB “decoy” oligodeoxynucleotides on IL-10 expression in LPS-induced mouse macrophages

目的 :探讨NF -κB“decoy”寡核苷酸 (ODNs)对脂多糖 (LPS)诱导小鼠巨噬细胞株J774 .1表达IL - 10的影响。方法 :通过体外细胞培养技术 ,观察转染NF -κB“decoy”ODNs对LPS刺激巨噬细胞产生IL - 10及其表达的影响。结果 :在LPS刺激的巨噬细胞中转染NF -κB“decoy”ODNs对抗炎介质IL - 10的合成表达无影响。结论 :NF-κB“decoy”ODNs不抑制抗炎介质IL - 10的表达 ,可能由于NF -κB在IL - 10转录机制中不占主导地位。

AIM: To study the effect of NF-κB “decoy”oligodeoxynucleotides on IL-10 expression in LPS-induced mouse macrophages. METHODS: Mouse macrophage cell line J774 1 cells were cultured with LPS and liposome-mediated oligodeoxynucleotides, and the level of IL-10 were measured in culture medium by enzyme linked immunosorbent assay. RNA was extracted from macrophages, and the mRNA expression of IL-10 were observed by RT-PCR. RESULTS: NF-κB “decoy”oligodeoxynucleotides did not inhibit the expression of IL-10 in LPS-induced macrophage, and did not reduce generation of IL-10. CONCLUSION: NF-κB decoy ODNs did not depress the mRNA expression of IL-10. NF-κB may not be the critical transcription factor in transduction mechanism of IL-10.